5F8B

Crystallographic Structure of PsoE with Co

Summary for 5F8B

• Entry DOI: 10.2210/pdb5f8b/pdb
• Descriptor: Glutathione S-transferase, putative, COBALT (II) ION, GLUTATHIONE, ... (5 entities in total)
• Functional Keywords: gst, transferase
• Biological source: Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
• Total number of polymer chains: 1
• Total formula weight: 28481.30

Authors

Hara, K.,Hashimoto, H.,Yamamoto, T.,Tsunematsu, Y.,Watanabe, K. (deposition date: 2015-12-09, release date: 2016-04-20, Last modification date: 2024-03-20)

Primary citation

Yamamoto, T.,Tsunematsu, Y.,Hara, K.,Suzuki, T.,Kishimoto, S.,Kawagishi, H.,Noguchi, H.,Hashimoto, H.,Tang, Y.,Hotta, K.,Watanabe, K.
Oxidative trans to cis Isomerization of Olefins in Polyketide Biosynthesis.
Angew. Chem. Int. Ed. Engl., 55:6207-6210, 2016

PubMed Abstract: Geometric isomerization can expand the scope of biological activities of natural products. The observed chemical diversity among the pseurotin-type fungal secondary metabolites is in part generated by a trans to cis isomerization of an olefin. In vitro characterizations of pseurotin biosynthetic enzymes revealed that the glutathione S-transferase PsoE requires participation of the bifunctional C-methyltransferase/epoxidase PsoF to complete the trans to cis isomerization of the pathway intermediate presynerazol. The crystal structure of the PsoE/glutathione/presynerazol complex indicated stereospecific glutathione-presynerazol conjugate formation is the principal function of PsoE. Moreover, PsoF was identified to have an additional, unexpected oxidative isomerase activity, thus making it a trifunctional enzyme which is key to the complexity generation in pseurotin biosynthesis. Through the study, we identified a novel mechanism of accomplishing a seemingly simple trans to cis isomerization reaction.

PubMed: 27072782
DOI: 10.1002/anie.201600940

Experimental method

X-RAY DIFFRACTION (2.54 Å)