5F7V

ABC substrate-binding protein Lmo0181 from Listeria monocytogenes in complex with cycloalternan

5F7V の概要

• エントリーDOI: 10.2210/pdb5f7v/pdb
• 関連するPDBエントリー: 5F7P 5F7Q 5F7R
• 分子名称: Lmo0181 protein, Cyclic alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: substrate-binding protein, structural genomics, center for structural genomics of infectious diseases, csgid, cycloalternan binding protein
• 由来する生物種: Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45458.99

構造登録者

Light, S.H.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2015-12-08, 公開日: 2016-09-07, 最終更新日: 2024-03-06)

主引用文献

Light, S.H.,Cahoon, L.A.,Halavaty, A.S.,Freitag, N.E.,Anderson, W.F.
Structure to function of an alpha-glucan metabolic pathway that promotes Listeria monocytogenes pathogenesis.
Nat Microbiol, 2:16202-16202, 2016

PubMed Abstract: Here we employ a 'systems structural biology' approach to functionally characterize an unconventional α-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.

PubMed: 27819654
DOI: 10.1038/nmicrobiol.2016.202

実験手法

X-RAY DIFFRACTION (1.4 Å)