5F6I

Crystal Structure of Tier 2 Neutralizing Antibody DH428 from a Rhesus Macaque

5F6I の概要

• エントリーDOI: 10.2210/pdb5f6i/pdb
• 関連するPDBエントリー: 5F6H 5F6J
• 分子名称: DH428 Antibody Heavy Chain, DH428 Antibody Light Chain (3 entities in total)
• 機能のキーワード: fab fragment, hiv-1, antibody, immune system
• 由来する生物種: Macaca mulatta; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47219.33

構造登録者

Fera, D.,Harrison, S.C. (登録日: 2015-12-06, 公開日: 2016-01-13, 最終更新日: 2024-10-23)

主引用文献

Bradley, T.,Fera, D.,Bhiman, J.,Eslamizar, L.,Lu, X.,Anasti, K.,Zhang, R.,Sutherland, L.L.,Scearce, R.M.,Bowman, C.M.,Stolarchuk, C.,Lloyd, K.E.,Parks, R.,Eaton, A.,Foulger, A.,Nie, X.,Karim, S.S.,Barnett, S.,Kelsoe, G.,Kepler, T.B.,Alam, S.M.,Montefiori, D.C.,Moody, M.A.,Liao, H.X.,Morris, L.,Santra, S.,Harrison, S.C.,Haynes, B.F.
Structural Constraints of Vaccine-Induced Tier-2 Autologous HIV Neutralizing Antibodies Targeting the Receptor-Binding Site.
Cell Rep, 14:43-54, 2016

PubMed Abstract: Antibodies that neutralize autologous transmitted/founder (TF) HIV occur in most HIV-infected individuals and can evolve to neutralization breadth. Autologous neutralizing antibodies (nAbs) against neutralization-resistant (Tier-2) viruses are rarely induced by vaccination. Whereas broadly neutralizing antibody (bnAb)-HIV-Envelope structures have been defined, the structures of autologous nAbs have not. Here, we show that immunization with TF mutant Envs gp140 oligomers induced high-titer, V5-dependent plasma neutralization for a Tier-2 autologous TF evolved mutant virus. Structural analysis of autologous nAb DH427 revealed binding to V5, demonstrating the source of narrow nAb specificity and explaining the failure to acquire breadth. Thus, oligomeric TF Envs can elicit autologous nAbs to Tier-2 HIVs, but induction of bnAbs will require targeting of precursors of B cell lineages that can mature to heterologous neutralization.

PubMed: 26725118
DOI: 10.1016/j.celrep.2015.12.017

実験手法

X-RAY DIFFRACTION (2.32 Å)