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5F0P

Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1(L557M) (SeMet labeled)

5F0P の概要
エントリーDOI10.2210/pdb5f0p/pdb
関連するPDBエントリー2FAU 2R17 5F0J 5F0K 5F0L 5F0M
分子名称Vacuolar protein sorting-associated protein 35, Vacuolar protein sorting-associated protein 26A, Sorting nexin-3, ... (9 entities in total)
機能のキーワードprotein transport, retromer, sorting nexin
由来する生物種Homo sapiens (Human)
詳細
細胞内の位置Cytoplasm: Q96QK1 O75436
Early endosome : O60493
Isoform 2: Cell membrane ; Multi- pass membrane protein . Endosome membrane ; Multi-pass membrane protein : P49281
タンパク質・核酸の鎖数4
化学式量合計114930.16
構造登録者
Lucas, M.,Gershlick, D.,Vidaurrazaga, A.,Rojas, A.L.,Bonifacino, J.S.,Hierro, A. (登録日: 2015-11-27, 公開日: 2016-12-07, 最終更新日: 2024-10-23)
主引用文献Lucas, M.,Gershlick, D.C.,Vidaurrazaga, A.,Rojas, A.L.,Bonifacino, J.S.,Hierro, A.
Structural Mechanism for Cargo Recognition by the Retromer Complex.
Cell, 167:1623-1635.e14, 2016
Cited by
PubMed Abstract: Retromer is a multi-protein complex that recycles transmembrane cargo from endosomes to the trans-Golgi network and the plasma membrane. Defects in retromer impair various cellular processes and underlie some forms of Alzheimer's disease and Parkinson's disease. Although retromer was discovered over 15 years ago, the mechanisms for cargo recognition and recruitment to endosomes have remained elusive. Here, we present an X-ray crystallographic analysis of a four-component complex comprising the VPS26 and VPS35 subunits of retromer, the sorting nexin SNX3, and a recycling signal from the divalent cation transporter DMT1-II. This analysis identifies a binding site for canonical recycling signals at the interface between VPS26 and SNX3. In addition, the structure highlights a network of cooperative interactions among the VPS subunits, SNX3, and cargo that couple signal-recognition to membrane recruitment.
PubMed: 27889239
DOI: 10.1016/j.cell.2016.10.056
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.78 Å)
構造検証レポート
Validation report summary of 5f0p
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-06に公開中

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