5EXB

Wild type green fluorescent protein DendFP (Dendronephthya sp.)

5EXB の概要

• エントリーDOI: 10.2210/pdb5exb/pdb
• 関連するPDBエントリー: 2VZX
• 分子名称: Green fluorescent protein, GLYCEROL (3 entities in total)
• 機能のキーワード: fluorescent protein, photoswitchable fluorescent proteins, chromophore, beta-barrel, biomarker
• 由来する生物種: Dendronephthya sp.
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 429930.54

構造登録者

Pletnev, V.Z.,Pletneva, N.V.,Pletnev, S.V. (登録日: 2015-11-23, 公開日: 2016-08-03, 最終更新日: 2024-11-06)

主引用文献

Pletneva, N.V.,Pletnev, S.,Pakhomov, A.A.,Chertkova, R.V.,Martynov, V.I.,Muslinkina, L.,Dauter, Z.,Pletnev, V.Z.
Crystal structure of the fluorescent protein from Dendronephthya sp. in both green and photoconverted red forms.
Acta Crystallogr D Struct Biol, 72:922-932, 2016

PubMed Abstract: The fluorescent protein from Dendronephthya sp. (DendFP) is a member of the Kaede-like group of photoconvertible fluorescent proteins with a His62-Tyr63-Gly64 chromophore-forming sequence. Upon irradiation with UV and blue light, the fluorescence of DendFP irreversibly changes from green (506 nm) to red (578 nm). The photoconversion is accompanied by cleavage of the peptide backbone at the C(α)-N bond of His62 and the formation of a terminal carboxamide group at the preceding Leu61. The resulting double C(α)=C(β) bond in His62 extends the conjugation of the chromophore π system to include imidazole, providing the red fluorescence. Here, the three-dimensional structures of native green and photoconverted red forms of DendFP determined at 1.81 and 2.14 Å resolution, respectively, are reported. This is the first structure of photoconverted red DendFP to be reported to date. The structure-based mutagenesis of DendFP revealed an important role of positions 142 and 193: replacement of the original Ser142 and His193 caused a moderate red shift in the fluorescence and a considerable increase in the photoconversion rate. It was demonstrated that hydrogen bonding of the chromophore to the Gln116 and Ser105 cluster is crucial for variation of the photoconversion rate. The single replacement Gln116Asn disrupts the hydrogen bonding of Gln116 to the chromophore, resulting in a 30-fold decrease in the photoconversion rate, which was partially restored by a further Ser105Asn replacement.

PubMed: 27487823
DOI: 10.1107/S205979831601038X

実験手法

X-RAY DIFFRACTION (1.81 Å)