5EUE

S1P Lyase Bacterial Surrogate bound to N-(2-((4-methoxy-2,5-dimethylbenzyl)amino)-1-phenylethyl)-5-methylisoxazole-3-carboxamide

5EUE の概要

• エントリーDOI: 10.2210/pdb5eue/pdb
• 関連するPDBエントリー: 5EUD
• 分子名称: Putative sphingosine-1-phosphate lyase, ~{N}-[(1~{S})-2-[(4-methoxy-2,5-dimethyl-phenyl)methylamino]-1-phenyl-ethyl]-5-methyl-1,2-oxazole-3-carboxamide, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: s1p lyase bacterial surrogate, lyase
• 由来する生物種: Symbiobacterium thermophilum (strain T / IAM 14863)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 112458.99

構造登録者

Argiriadi, M.A.,Banach, D.,Radziejewska, E.,Marchie, S.,DiMauro, J.,Dinges, J.,Dominguez, E.,Hutchins, C.,Judge, R.A.,Queeney, K.,Wallace, G.,Harris, C.M. (登録日: 2015-11-18, 公開日: 2016-03-16, 最終更新日: 2023-11-15)

主引用文献

Argiriadi, M.A.,Banach, D.,Radziejewska, E.,Marchie, S.,DiMauro, J.,Dinges, J.,Dominguez, E.,Hutchins, C.,Judge, R.A.,Queeney, K.,Wallace, G.,Harris, C.M.
Creation of a S1P Lyase bacterial surrogate for structure-based drug design.
Bioorg.Med.Chem.Lett., 26:2293-2296, 2016

PubMed Abstract: S1P Lyase (SPL) has been described as a drug target in the treatment of autoimmune diseases. It plays an important role in maintaining intracellular levels of S1P thereby affecting T cell egress from lymphoid tissues. Several groups have already published approaches to inhibit S1P Lyase with small molecules, which in turn increase endogenous S1P concentrations resulting in immunosuppression. The use of structural biology has previously aided SPL inhibitor design. Novel construct design is at times necessary to provide a reagent for protein crystallography. Here we present a chimeric bacterial protein scaffold used for protein X-ray structures in the presence of early small molecule inhibitors. Mutations were introduced to the bacterial SPL from Symbiobacterium thermophilum which mimic the human enzyme. As a result, two mutant StSPL crystal structures resolved to 2.8Å and 2.2Å resolutions were solved and provide initial structural hypotheses for an isoxazole chemical series, whose optimization is discussed in the accompanying paper.

PubMed: 27013389
DOI: 10.1016/j.bmcl.2016.02.084

実験手法

X-RAY DIFFRACTION (2.83 Å)