5ERR

GephE in complex with Mg(2+) - ADP

5ERR の概要

• エントリーDOI: 10.2210/pdb5err/pdb
• 分子名称: Gephyrin, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: molybdenum and tungsten cofactor, moco, wco, terminal step, transferase
• 由来する生物種: Rattus norvegicus (Norway Rat)
• 細胞内の位置: Cell junction, synapse : Q03555
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47050.22

構造登録者

Kasaragod, V.B.,Schindelin, H. (登録日: 2015-11-15, 公開日: 2016-05-04, 最終更新日: 2024-01-10)

主引用文献

Kasaragod, V.B.,Schindelin, H.
Structural Framework for Metal Incorporation during Molybdenum Cofactor Biosynthesis.
Structure, 24:782-788, 2016

PubMed Abstract: The molybdenum cofactor (Moco) is essential for the catalytic activity of all molybdenum-containing enzymes with the exception of nitrogenase. Moco biosynthesis follows an evolutionarily highly conserved pathway and genetic deficiencies in the corresponding human enzymes result in Moco deficiency, which manifests itself in severe neurological symptoms and death in childhood. In humans the final steps of Moco biosynthesis are catalyzed by gephyrin, specifically the penultimate adenylation of molybdopterin (MPT) by its N-terminal G domain (GephG) and the final metal incorporation by its C-terminal E domain (GephE). To better understand the poorly defined molecular framework of this final step, we determined high-resolution crystal structures of GephE in the apo state and in complex with ADP, AMP, and molybdate. Our data provide novel insights into the catalytic steps leading to final Moco maturation, namely deadenylation as well as molybdate binding and insertion.

PubMed: 27112598
DOI: 10.1016/j.str.2016.02.023

実験手法

X-RAY DIFFRACTION (1.65 Å)