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5ERC

X-ray crystal structure of BRPF1 PZP domain

Summary for 5ERC
Entry DOI10.2210/pdb5erc/pdb
DescriptorPeregrin, ZINC ION, CALCIUM ION, ... (4 entities in total)
Functional Keywordsreader domain, methyllysine, dna, h3 histone, transcription
Biological sourceHomo sapiens (Human)
Total number of polymer chains1
Total formula weight20035.84
Authors
Klein, B.J.,Andrews, F.H.,Kutateladze, T.G. (deposition date: 2015-11-13, release date: 2015-12-30, Last modification date: 2024-03-06)
Primary citationKlein, B.J.,Muthurajan, U.M.,Lalonde, M.E.,Gibson, M.D.,Andrews, F.H.,Hepler, M.,Machida, S.,Yan, K.,Kurumizaka, H.,Poirier, M.G.,Cote, J.,Luger, K.,Kutateladze, T.G.
Bivalent interaction of the PZP domain of BRPF1 with the nucleosome impacts chromatin dynamics and acetylation.
Nucleic Acids Res., 44:472-484, 2016
Cited by
PubMed Abstract: BRPF1 (bromodomain PHD finger 1) is a core subunit of the MOZ histone acetyltransferase (HAT) complex, critical for normal developmental programs and implicated in acute leukemias. BRPF1 contains a unique assembly of zinc fingers, termed a PZP domain, the physiological role of which remains unclear. Here, we elucidate the structure-function relationship of this novel epigenetic reader and detail the biological and mechanistic consequences of its interaction with nucleosomes. PZP has a globular architecture and forms a 2:1 stoichiometry complex with the nucleosome, bivalently interacting with histone H3 and DNA. This binding impacts the nucleosome dynamics, shifting the DNA unwrapping/rewrapping equilibrium toward the unwrapped state and increasing DNA accessibility. We demonstrate that the DNA-binding function of the BRPF1 PZP domain is required for the MOZ-BRPF1-ING5-hEaf6 HAT complex to be recruited to chromatin and to acetylate nucleosomal histones. Our findings reveal a novel link between chromatin dynamics and MOZ-mediated acetylation.
PubMed: 26626149
DOI: 10.1093/nar/gkv1321
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.05 Å)
Structure validation

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数据于2025-10-15公开中

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