5EOS

Crystallizing Strained DNA: Self-Assembly of 3D Crystals Containing a Torsionally-Stressed Component

5EOS の概要

• エントリーDOI: 10.2210/pdb5eos/pdb
• 関連するPDBエントリー: 3GBI
• 分子名称: DNA (5'-D(P*GP*AP*GP*CP*AP*GP*CP*TP*GP*TP*G)-3'), DNA (5'-D(P*CP*AP*CP*AP*CP*CP*GP*T)-3'), DNA (5'-D(*TP*CP*TP*GP*AP*TP*GP*TP*GP*CP*TP*GP*C)-3'), ... (4 entities in total)
• 機能のキーワード: dna nanotechnology, torsionally strained dna, dna lattices, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12797.42

構造登録者

Hernandez, C.,Birktoft, J.J.,Seeman, N.C. (登録日: 2015-11-10, 公開日: 2017-08-09, 最終更新日: 2024-03-06)

主引用文献

Hernandez, C.,Birktoft, J.J.,Ohayon, Y.P.,Chandrasekaran, A.R.,Abdallah, H.,Sha, R.,Stojanoff, V.,Mao, C.,Seeman, N.C.
Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component.
Cell Chem Biol, 2017

PubMed Abstract: There is an increasing appreciation for structural diversity of DNA that is of interest to both DNA nanotechnology and basic biology. Here, we have explored how DNA responds to torsional stress by building on a previously reported two-turn DNA tensegrity triangle and demonstrating that we could introduce an extra nucleotide pair (np) into the original sequence without affecting assembly and crystallization. The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice. The work reveals a near-atomic structure of naked DNA under a torsional stress of approximately 14%, and thus provides an example of DNA distortions that occur without a requirement for either an external energy source or the free energy available from protein or drug binding.

PubMed: 28988948
DOI: 10.1016/j.chembiol.2017.08.018

実験手法

X-RAY DIFFRACTION (4.7 Å)