5EO7

Crystal structure of AOL

Summary for 5EO7

• Entry DOI: 10.2210/pdb5eo7/pdb
• Related: 5EO8
• Descriptor: Predicted protein, methyl 1-seleno-alpha-L-fucopyranoside, SULFATE ION, ... (4 entities in total)
• Functional Keywords: seleno-fucoses, phaing, lectin, aspergillus oryzae, sugar binding protein
• Biological source: Aspergillus oryzae RIB40 (Yellow koji mold)
• Total number of polymer chains: 3
• Total formula weight: 107334.73

Authors

Kato, R.,Kiso, M.,Ishida, H.,Ando, H.,Suzuki, T.,Shimabukuro, S.,Makyio, H. (deposition date: 2015-11-10, release date: 2016-06-29, Last modification date: 2024-03-20)

Primary citation

Makyio, H.,Shimabukuro, J.,Suzuki, T.,Imamura, A.,Ishida, H.,Kiso, M.,Ando, H.,Kato, R.
Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin
Biochem.Biophys.Res.Commun., 477:477-482, 2016

PubMed Abstract: The crystal structure of AOL (a fucose-specific lectin of Aspergillus oryzae) has been solved by SAD (single-wavelength anomalous diffraction) and MAD (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. The overall structure is a six-bladed β-propeller similar to that of other fucose-specific lectins. The fucose moieties of the seleno-fucosides are located in six fucose-binding sites. Although the Arg and Glu/Gln residues bound to the fucose moiety are common to all fucose-binding sites, the amino-acid residues involved in fucose binding at each site are not identical. The varying peak heights of the seleniums in the electron density map suggest that each fucose-binding site has a different carbohydrate binding affinity.

PubMed: 27318092
DOI: 10.1016/j.bbrc.2016.06.069

Experimental method

X-RAY DIFFRACTION (2.3 Å)