5ELD

Cholera toxin classical B-pentamer in complex with A Lewis-y

Summary for 5ELD

• Entry DOI: 10.2210/pdb5eld/pdb
• Related PRD ID: PRD_900128
• Descriptor: Cholera enterotoxin B subunit, alpha-L-fucopyranose-(1-2)-[2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)]beta-D-galactopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-alpha-D-glucopyranose, DI(HYDROXYETHYL)ETHER, ... (9 entities in total)
• Functional Keywords: cholera toxin b-pentamer, a lewis-y, complex, blood group oligosaccharide/antigen, toxin
• Biological source: Vibrio cholerae O1
• Total number of polymer chains: 5
• Total formula weight: 63233.24

Authors

Heggelund, J.E.,Burschowsky, D.,Krengel, U. (deposition date: 2015-11-04, release date: 2016-03-30, Last modification date: 2024-10-23)

Primary citation

Heggelund, J.E.,Burschowsky, D.,Bjrnestad, V.A.,Hodnik, V.,Anderluh, G.,Krengel, U.
High-Resolution Crystal Structures Elucidate the Molecular Basis of Cholera Blood Group Dependence.
Plos Pathog., 12:e1005567-e1005567, 2016

PubMed Abstract: Cholera is the prime example of blood-group-dependent diseases, with individuals of blood group O experiencing the most severe symptoms. The cholera toxin is the main suspect to cause this relationship. We report the high-resolution crystal structures (1.1-1.6 Å) of the native cholera toxin B-pentamer for both classical and El Tor biotypes, in complexes with relevant blood group determinants and a fragment of its primary receptor, the GM1 ganglioside. The blood group A determinant binds in the opposite orientation compared to previously published structures of the cholera toxin, whereas the blood group H determinant, characteristic of blood group O, binds in both orientations. H-determinants bind with higher affinity than A-determinants, as shown by surface plasmon resonance. Together, these findings suggest why blood group O is a risk factor for severe cholera.

PubMed: 27082955
DOI: 10.1371/journal.ppat.1005567

Experimental method

X-RAY DIFFRACTION (1.4 Å)