5EFW

Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1

5EFW の概要

• エントリーDOI: 10.2210/pdb5efw/pdb
• 関連するPDBエントリー: 1LP1 2V0U
• 分子名称: NPH1-1, Z-dark, a small protein based on the Z domain affibody, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: lov domain, photoreceptor, affibody, optogenetic tool, signaling protein
• 由来する生物種: Avena sativa (Oat); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 30795.12

構造登録者

Winkler, A.,Wang, H.,Hartmann, E.,Hahn, K.,Schlichting, I. (登録日: 2015-10-26, 公開日: 2016-07-20, 最終更新日: 2024-01-10)

主引用文献

Wang, H.,Vilela, M.,Winkler, A.,Tarnawski, M.,Schlichting, I.,Yumerefendi, H.,Kuhlman, B.,Liu, R.,Danuser, G.,Hahn, K.M.
LOVTRAP: an optogenetic system for photoinduced protein dissociation.
Nat.Methods, 13:755-758, 2016

PubMed Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

PubMed: 27427858
DOI: 10.1038/nmeth.3926

実験手法

X-RAY DIFFRACTION (2.1 Å)