5EEX

RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 9.02 MGy

5EEX の概要

• エントリーDOI: 10.2210/pdb5eex/pdb
• 関連するPDBエントリー: 1gtf 5eeu
• 分子名称: Transcription attenuation protein MtrB, (GAGUU)10GAG 53-NUCLEOTIDE RNA, TRYPTOPHAN, ... (4 entities in total)
• 機能のキーワード: protein-rna complex, radiation damage, rna binding protein
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 204061.72

構造登録者

Bury, C.S.,McGeehan, J.E.,Garman, E.F.,Shevtsov, M.B. (登録日: 2015-10-23, 公開日: 2016-05-04, 最終更新日: 2024-01-10)

主引用文献

Bury, C.S.,McGeehan, J.E.,Antson, A.A.,Carmichael, I.,Gerstel, M.,Shevtsov, M.B.,Garman, E.F.
RNA protects a nucleoprotein complex against radiation damage.
Acta Crystallogr D Struct Biol, 72:648-657, 2016

PubMed Abstract: Radiation damage during macromolecular X-ray crystallographic data collection is still the main impediment for many macromolecular structure determinations. Even when an eventual model results from the crystallographic pipeline, the manifestations of radiation-induced structural and conformation changes, the so-called specific damage, within crystalline macromolecules can lead to false interpretations of biological mechanisms. Although this has been well characterized within protein crystals, far less is known about specific damage effects within the larger class of nucleoprotein complexes. Here, a methodology has been developed whereby per-atom density changes could be quantified with increasing dose over a wide (1.3-25.0 MGy) range and at higher resolution (1.98 Å) than the previous systematic specific damage study on a protein-DNA complex. Specific damage manifestations were determined within the large trp RNA-binding attenuation protein (TRAP) bound to a single-stranded RNA that forms a belt around the protein. Over a large dose range, the RNA was found to be far less susceptible to radiation-induced chemical changes than the protein. The availability of two TRAP molecules in the asymmetric unit, of which only one contained bound RNA, allowed a controlled investigation into the exact role of RNA binding in protein specific damage susceptibility. The 11-fold symmetry within each TRAP ring permitted statistically significant analysis of the Glu and Asp damage patterns, with RNA binding unexpectedly being observed to protect these otherwise highly sensitive residues within the 11 RNA-binding pockets distributed around the outside of the protein molecule. Additionally, the method enabled a quantification of the reduction in radiation-induced Lys and Phe disordering upon RNA binding directly from the electron density.

PubMed: 27139628
DOI: 10.1107/S2059798316003351

実験手法

X-RAY DIFFRACTION (1.98 Å)