5EE0
Crystal structure of OsYchF1 at pH 6.5
Summary for 5EE0
| Entry DOI | 10.2210/pdb5ee0/pdb |
| Related | 5EE1 5EE3 5EE9 |
| Descriptor | Obg-like ATPase 1 (2 entities in total) |
| Functional Keywords | osychf1, gtp-binding protein, amp-pnp, ychf-type, p-loop ntpase, atp-binding protein, hydrolase |
| Biological source | Oryza sativa subsp. japonica (Rice) |
| Total number of polymer chains | 1 |
| Total formula weight | 44485.89 |
| Authors | |
| Primary citation | Cheung, M.-Y.,Li, X.,Miao, R.,Fong, Y.-H.,Li, K.-P.,Yung, Y.-L.,Yu, M.-H.,Wong, K.-B.,Chen, Z.,Lam, H.-M. ATP binding by the P-loop NTPase OsYchF1 (an unconventional G protein) contributes to biotic but not abiotic stress responses Proc.Natl.Acad.Sci.USA, 113:2648-2653, 2016 Cited by PubMed Abstract: G proteins are involved in almost all aspects of the cellular regulatory pathways through their ability to bind and hydrolyze GTP. The YchF subfamily, interestingly, possesses the unique ability to bind both ATP and GTP, and is possibly an ancestral form of G proteins based on phylogenetic studies and is present in all kingdoms of life. However, the biological significance of such a relaxed ligand specificity has long eluded researchers. Here, we have elucidated the different conformational changes caused by the binding of a YchF homolog in rice (OsYchF1) to ATP versus GTP by X-ray crystallography. Furthermore, by comparing the 3D relationships of the ligand position and the various amino acid residues at the binding sites in the crystal structures of the apo-bound and ligand-bound versions, a mechanism for the protein's ability to bind both ligands is revealed. Mutation of the noncanonical G4 motif of the OsYchF1 to the canonical sequence for GTP specificity precludes the binding/hydrolysis of ATP and prevents OsYchF1 from functioning as a negative regulator of plant-defense responses, while retaining its ability to bind/hydrolyze GTP and its function as a negative regulator of abiotic stress responses, demonstrating the specific role of ATP-binding/hydrolysis in disease resistance. This discovery will have a significant impact on our understanding of the structure-function relationships of the YchF subfamily of G proteins in all kingdoms of life. PubMed: 26912459DOI: 10.1073/pnas.1522966113 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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