5ED8

Crystal structure of CC2-SUN of mouse SUN2

5ED8 の概要

• エントリーDOI: 10.2210/pdb5ed8/pdb
• 分子名称: MKIAA0668 protein, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: sun domain, nuclear membrane, transport protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30075.21

構造登録者

Nie, S.,Ke, H.M.,Gao, F.,Ren, J.Q.,Wang, M.Z.,Huo, L.,Gong, W.M.,Feng, W. (登録日: 2015-10-21, 公開日: 2016-01-13, 最終更新日: 2024-03-20)

主引用文献

Nie, S.,Ke, H.,Gao, F.,Ren, J.,Wang, M.,Huo, L.,Gong, W.,Feng, W.
Coiled-Coil Domains of SUN Proteins as Intrinsic Dynamic Regulators
Structure, 24:80-91, 2016

PubMed Abstract: SUN proteins are the core components of LINC complexes that span across the nuclear envelope for nuclear positioning and migration. SUN proteins contain at least one predicted coiled-coil domain preceding the SUN domain. Here, we found that the two coiled-coil domains (CC1 and CC2) of SUN2 exhibit distinct oligomeric states. CC2 is a monomer in solution. The structure of the CC2-SUN monomer revealed that CC2 unexpectedly folds as a three-helix bundle that interacts with the SUN domain and locks it in an inactive conformation. In contrast, CC1 is a trimer. The structure of the CC1 trimer demonstrated that CC1 is an imperfect coiled coil for the trimerization and activation of the SUN domain. Modulations of CC1 and CC2 dictate different oligomeric states of CC1-CC2-SUN, which is essential for LINC complex formation. Thus, the two coiled-coil domains of SUN2 act as the intrinsic dynamic regulators for controlling the SUN domain activity.

PubMed: 26688217
DOI: 10.1016/j.str.2015.10.024

実験手法

X-RAY DIFFRACTION (2.5 Å)