5E8B

Crystal structure of the S. cerevisiae Rtf1 histone modification domain mutant R126A

5E8B の概要

• エントリーDOI: 10.2210/pdb5e8b/pdb
• 関連するPDBエントリー: 5EMX
• 分子名称: RNA polymerase-associated protein RTF1, SODIUM ION, BENZAMIDINE, ... (4 entities in total)
• 機能のキーワード: transcription, paf1, chromatin, rtf1
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• 細胞内の位置: Nucleus, nucleoplasm : P53064
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16479.22

構造登録者

Wier, A.D.,Heroux, A.,VanDemark, A.P. (登録日: 2015-10-14, 公開日: 2016-11-09, 最終更新日: 2023-09-27)

主引用文献

Van Oss, S.B.,Shirra, M.K.,Bataille, A.R.,Wier, A.D.,Yen, K.,Vinayachandran, V.,Byeon, I.L.,Cucinotta, C.E.,Heroux, A.,Jeon, J.,Kim, J.,VanDemark, A.P.,Pugh, B.F.,Arndt, K.M.
The Histone Modification Domain of Paf1 Complex Subunit Rtf1 Directly Stimulates H2B Ubiquitylation through an Interaction with Rad6.
Mol. Cell, 64:815-825, 2016

PubMed Abstract: The five-subunit yeast Paf1 complex (Paf1C) regulates all stages of transcription and is critical for the monoubiquitylation of histone H2B (H2Bub), a modification that broadly influences chromatin structure and eukaryotic transcription. Here, we show that the histone modification domain (HMD) of Paf1C subunit Rtf1 directly interacts with the ubiquitin conjugase Rad6 and stimulates H2Bub independently of transcription. We present the crystal structure of the Rtf1 HMD and use site-specific, in vivo crosslinking to identify a conserved Rad6 interaction surface. Utilizing ChIP-exo analysis, we define the localization patterns of the H2Bub machinery at high resolution and demonstrate the importance of Paf1C in targeting the Rtf1 HMD, and thereby H2Bub, to its appropriate genomic locations. Finally, we observe HMD-dependent stimulation of H2Bub in a transcription-free, reconstituted in vitro system. Taken together, our results argue for an active role for Paf1C in promoting H2Bub and ensuring its proper localization in vivo.

PubMed: 27840029
DOI: 10.1016/j.molcel.2016.10.008

実験手法

X-RAY DIFFRACTION (1.62 Å)