5E41

Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 5-(N-(10-hydroxydecanoyl)-aminopentenyl)-2'-deoxyuridine-triphosphate

5E41 の概要

• エントリーDOI: 10.2210/pdb5e41/pdb
• 分子名称: DNA polymerase I, thermostable, DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3'), DNA (5'-D(*AP*AP*AP*AP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3'), ... (7 entities in total)
• 機能のキーワード: linker-modified nucleotide, klentaq, dna polymerase, transferase
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 70641.06

構造登録者

Hottin, A.,Betz, K.,Marx, A. (登録日: 2015-10-05, 公開日: 2016-12-07, 最終更新日: 2024-01-10)

主引用文献

Hottin, A.,Betz, K.,Diederichs, K.,Marx, A.
Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides.
Chemistry, 23:2109-2118, 2017

PubMed Abstract: Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and deoxyuridine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides that prove to be superior substrates for KlenTaq DNA polymerase compared with their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.

PubMed: 27901305
DOI: 10.1002/chem.201604515

実験手法

X-RAY DIFFRACTION (1.8 Å)