5E1K

Selenomethionine Ca2+-Calmodulin from Paramecium tetraurelia SAD data

5E1K の概要

• エントリーDOI: 10.2210/pdb5e1k/pdb
• 関連するPDBエントリー: 5E1N 5E1P
• 分子名称: Calmodulin, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: calcium signaling, ef hand, calcium binding, metal binding protein
• 由来する生物種: Paramecium tetraurelia
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17499.32

構造登録者

Lin, J.,van den Bedem, H.,Brunger, A.T.,Wilson, M.A. (登録日: 2015-09-29, 公開日: 2015-11-25, 最終更新日: 2024-10-16)

主引用文献

Lin, J.,van den Bedem, H.,Brunger, A.T.,Wilson, M.A.
Atomic resolution experimental phase information reveals extensive disorder and bound 2-methyl-2,4-pentanediol in Ca(2+)-calmodulin.
Acta Crystallogr D Struct Biol, 72:83-92, 2016

PubMed Abstract: Calmodulin (CaM) is the primary calcium signaling protein in eukaryotes and has been extensively studied using various biophysical techniques. Prior crystal structures have noted the presence of ambiguous electron density in both hydrophobic binding pockets of Ca(2+)-CaM, but no assignment of these features has been made. In addition, Ca(2+)-CaM samples many conformational substates in the crystal and accurately modeling the full range of this functionally important disorder is challenging. In order to characterize these features in a minimally biased manner, a 1.0 Å resolution single-wavelength anomalous diffraction data set was measured for selenomethionine-substituted Ca(2+)-CaM. Density-modified electron-density maps enabled the accurate assignment of Ca(2+)-CaM main-chain and side-chain disorder. These experimental maps also substantiate complex disorder models that were automatically built using low-contour features of model-phased electron density. Furthermore, experimental electron-density maps reveal that 2-methyl-2,4-pentanediol (MPD) is present in the C-terminal domain, mediates a lattice contact between N-terminal domains and may occupy the N-terminal binding pocket. The majority of the crystal structures of target-free Ca(2+)-CaM have been derived from crystals grown using MPD as a precipitant, and thus MPD is likely to be bound in functionally critical regions of Ca(2+)-CaM in most of these structures. The adventitious binding of MPD helps to explain differences between the Ca(2+)-CaM crystal and solution structures and is likely to favor more open conformations of the EF-hands in the crystal.

PubMed: 26894537
DOI: 10.1107/S2059798315021609

実験手法

X-RAY DIFFRACTION (1 Å)