5DZC

Crystal structure of the cGMP-dependent protein kinase PKG from Plasmodium Vivax - AMPPNP bound

5DZC の概要

• エントリーDOI: 10.2210/pdb5dzc/pdb
• 関連するPDBエントリー: 4MYI 4WJC
• 分子名称: cGMP-dependent protein kinase, putative, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, UNKNOWN ATOM OR ION, ... (6 entities in total)
• 機能のキーワード: structural genomics consortium (sgc), kinase, transferase
• 由来する生物種: Plasmodium vivax (strain Salvador I)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 97362.19

構造登録者

Walker, J.R.,El Bakkouri, M.,Loppnau, P.,Graslund, S.,He, H.,Seitova, A.,Hutchinson, A.,Bountra, C.,Edwards, A.M.,Arrowsmith, C.H.,Hui, R.,Amani, M.,Structural Genomics Consortium (SGC) (登録日: 2015-09-25, 公開日: 2015-10-14, 最終更新日: 2023-09-27)

主引用文献

El Bakkouri, M.,Kouidmi, I.,Wernimont, A.K.,Amani, M.,Hutchinson, A.,Loppnau, P.,Kim, J.J.,Flueck, C.,Walker, J.R.,Seitova, A.,Senisterra, G.,Kakihara, Y.,Kim, C.,Blackman, M.J.,Calmettes, C.,Baker, D.A.,Hui, R.
Structures of the cGMP-dependent protein kinase in malaria parasites reveal a unique structural relay mechanism for activation.
Proc.Natl.Acad.Sci.USA, 116:14164-14173, 2019

PubMed Abstract: The cyclic guanosine-3',5'-monophosphate (cGMP)-dependent protein kinase (PKG) was identified >25 y ago; however, efforts to obtain a structure of the entire PKG enzyme or catalytic domain from any species have failed. In malaria parasites, cooperative activation of PKG triggers crucial developmental transitions throughout the complex life cycle. We have determined the cGMP-free crystallographic structures of PKG from and , revealing how key structural components, including an N-terminal autoinhibitory segment (AIS), four predicted cyclic nucleotide-binding domains (CNBs), and a kinase domain (KD), are arranged when the enzyme is inactive. The four CNBs and the KD are in a pentagonal configuration, with the AIS docked in the substrate site of the KD in a swapped-domain dimeric arrangement. We show that although the protein is predominantly a monomer (the dimer is unlikely to be representative of the physiological form), the binding of the AIS is necessary to keep PKG inactive. A major feature is a helix serving the dual role of the N-terminal helix of the KD as well as the capping helix of the neighboring CNB. A network of connecting helices between neighboring CNBs contributes to maintaining the kinase in its inactive conformation. We propose a scheme in which cooperative binding of cGMP, beginning at the CNB closest to the KD, transmits conformational changes around the pentagonal molecule in a structural relay mechanism, enabling PKG to orchestrate rapid, highly regulated developmental switches in response to dynamic modulation of cGMP levels in the parasite.

PubMed: 31239348
DOI: 10.1073/pnas.1905558116

実験手法

X-RAY DIFFRACTION (2.3 Å)