5DUM

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 65C6

5DUM の概要

• エントリーDOI: 10.2210/pdb5dum/pdb
• 関連するPDBエントリー: 5DUP 5DUR 5DUT
• 分子名称: 65C6 Heavy Chain, 65C6 Light Chain, Hemagglutinin, ... (4 entities in total)
• 機能のキーワード: influenza virus, antibody, complex, neutralize, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 76106.96

構造登録者

Sun, J.,Zuo, T.,Wang, G.,Zhou, P.,Zhang, L.,Wang, X. (登録日: 2015-09-19, 公開日: 2015-12-16, 最終更新日: 2024-11-06)

主引用文献

Zuo, T.,Sun, J.,Wang, G.,Jiang, L.,Zuo, Y.,Li, D.,Shi, X.,Liu, X.,Fan, S.,Ren, H.,Hu, H.,Sun, L.,Zhou, B.,Liang, M.,Zhou, P.,Wang, X.,Zhang, L.
Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection
Nat Commun, 6:8855-8855, 2015

PubMed Abstract: Understanding the mechanism of protective antibody recognition against highly pathogenic avian influenza A virus H5N1 in humans is critical for the development of effective therapies and vaccines. Here we report the crystal structure of three H5-specific human monoclonal antibodies bound to the globular head of hemagglutinin (HA) with distinct epitope specificities, neutralization potencies and breadth. A structural and functional analysis of these epitopes combined with those reported elsewhere identifies four major vulnerable sites on the globular head of H5N1 HA. Chimeric and vulnerable site-specific mutant pseudoviruses are generated to delineate broad neutralization specificities of convalescent sera from two individuals who recovered from the infection with H5N1 virus. Our results show that the four vulnerable sites on the globular head rather than the stem region are the major neutralizing targets, suggesting that during natural H5N1 infection neutralizing antibodies against the globular head work in concert to provide protective antibody-mediated immunity.

PubMed: 26635249
DOI: 10.1038/ncomms9855

実験手法

X-RAY DIFFRACTION (3.003 Å)