5DTU

Crystal structure of the RNA-helicase Prp28 from Chaetomium thermophilum bound to ADP

5DTU の概要

• エントリーDOI: 10.2210/pdb5dtu/pdb
• 分子名称: Prp28, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: dead-box protein, atpase, rna-helicase, ddx23, hydrolase
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53415.10

構造登録者

Tauchert, M.J.,Ficner, R. (登録日: 2015-09-18, 公開日: 2016-05-04, 最終更新日: 2024-01-10)

主引用文献

Tauchert, M.J.,Ficner, R.
Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum.
Acta Crystallogr.,Sect.F, 72:409-416, 2016

PubMed Abstract: Prp28 (pre-mRNA-splicing ATP-dependent RNA helicase 28) is a spliceosomal DEAD-box helicase which is involved in two steps of spliceosome assembly. It is required for the formation of commitment complex 2 in an ATP-independent manner as well as for the formation of the pre-catalytic spliceosome, which in contrast is ATP-dependent. During the latter step, Prp28 is crucial for the integration of the U4/U6·U5 tri-snRNP since it displaces the U1 snRNP and allows the U6 snRNP to base-pair with the 5'-splice site. Here, the crystal structure of Prp28 from the thermophilic fungus Chaetomium thermophilum is reported at 3.2 Å resolution and is compared with the available structures of homologues.

PubMed: 27139834
DOI: 10.1107/S2053230X16006038

実験手法

X-RAY DIFFRACTION (3.199 Å)