5DPH

sfGFP mutant - 149 p-cyano-L-phenylalanine

5DPH の概要

• エントリーDOI: 10.2210/pdb5dph/pdb
• 関連するPDBエントリー: 5DPG 5DPI 5DPJ
• 分子名称: Green fluorescent protein, CARBON DIOXIDE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: gfp, unnatural amino acid, cyanophenylalanine, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54170.97

構造登録者

Dippel, A.B.,Olenginski, G.M.,Maurici, N.,Liskov, M.T.,Brewer, S.H.,Phillips-Piro, C.M. (登録日: 2015-09-12, 公開日: 2016-01-13, 最終更新日: 2023-11-15)

主引用文献

Dippel, A.B.,Olenginski, G.M.,Maurici, N.,Liskov, M.T.,Brewer, S.H.,Phillips-Piro, C.M.
Probing the effectiveness of spectroscopic reporter unnatural amino acids: a structural study.
Acta Crystallogr D Struct Biol, 72:121-130, 2016

PubMed Abstract: The X-ray crystal structures of superfolder green fluorescent protein (sfGFP) containing the spectroscopic reporter unnatural amino acids (UAAs) 4-cyano-L-phenylalanine (pCNF) or 4-ethynyl-L-phenylalanine (pCCF) at two unique sites in the protein have been determined. These UAAs were genetically incorporated into sfGFP in a solvent-exposed loop region and/or a partially buried site on the β-barrel of the protein. The crystal structures containing the UAAs at these two sites permit the structural implications of UAA incorporation for the native protein structure to be assessed with high resolution and permit a direct correlation between the structure and spectroscopic data to be made. The structural implications were quantified by comparing the root-mean-square deviation (r.m.s.d.) between the crystal structure of wild-type sfGFP and the protein constructs containing either pCNF or pCCF in the local environment around the UAAs and in the overall protein structure. The results suggest that the selective placement of these spectroscopic reporter UAAs permits local protein environments to be studied in a relatively nonperturbative fashion with site-specificity.

PubMed: 26894540
DOI: 10.1107/S2059798315022858

実験手法

X-RAY DIFFRACTION (1.42 Å)