5DOI

Crystal structure of Tetrahymena p45N and p19

Summary for 5DOI

• Entry DOI: 10.2210/pdb5doi/pdb
• Related: 5DOF 5DOK
• Descriptor: Telomerase-associated protein 19, Telomerase associated protein p45 (3 entities in total)
• Functional Keywords: telomerase, ob fold, tetrahymena, dna binding protein
• Biological source: Tetrahymena thermophila; More
• Total number of polymer chains: 8
• Total formula weight: 136019.27

Authors

Wan, B.,Tang, T.,Wu, J.,Lei, M. (deposition date: 2015-09-11, release date: 2015-11-25, Last modification date: 2024-03-20)

Primary citation

Wan, B.,Tang, T.,Upton, H.,Shuai, J.,Zhou, Y.,Li, S.,Chen, J.,Brunzelle, J.S.,Zeng, Z.,Collins, K.,Wu, J.,Lei, M.
The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex
Nat.Struct.Mol.Biol., 22:1023-1026, 2015

PubMed Abstract: Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3'-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.

PubMed: 26551074
DOI: 10.1038/nsmb.3126

Experimental method

X-RAY DIFFRACTION (2.2 Å)