5DOI

Crystal structure of Tetrahymena p45N and p19

5DOI の概要

• エントリーDOI: 10.2210/pdb5doi/pdb
• 関連するPDBエントリー: 5DOF 5DOK
• 分子名称: Telomerase-associated protein 19, Telomerase associated protein p45 (3 entities in total)
• 機能のキーワード: telomerase, ob fold, tetrahymena, dna binding protein
• 由来する生物種: Tetrahymena thermophila; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 136019.27

構造登録者

Wan, B.,Tang, T.,Wu, J.,Lei, M. (登録日: 2015-09-11, 公開日: 2015-11-25, 最終更新日: 2024-03-20)

主引用文献

Wan, B.,Tang, T.,Upton, H.,Shuai, J.,Zhou, Y.,Li, S.,Chen, J.,Brunzelle, J.S.,Zeng, Z.,Collins, K.,Wu, J.,Lei, M.
The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex
Nat.Struct.Mol.Biol., 22:1023-1026, 2015

PubMed Abstract: Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3'-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.

PubMed: 26551074
DOI: 10.1038/nsmb.3126

実験手法

X-RAY DIFFRACTION (2.2 Å)