Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

5DLJ

Crystal Structure of Cas-DNA-N1 complex

5DLJ の概要
エントリーDOI10.2210/pdb5dlj/pdb
関連するPDBエントリー5DQT 5DQU 5DQZ
分子名称CRISPR-associated endonuclease Cas1, 39-mer DNA N1-F, 39-mer DNA N1-R, ... (5 entities in total)
機能のキーワードprotein-dna complex, hydrolase-dna complex, hydrolase/dna
由来する生物種Escherichia coli K12
詳細
細胞内の位置Cytoplasm : Q46896
タンパク質・核酸の鎖数8
化学式量合計164304.00
構造登録者
Wang, J.,Li, J.,Zhao, H.,Sheng, G.,Wang, M.,Yin, M.,Wang, Y. (登録日: 2015-09-05, 公開日: 2015-11-11, 最終更新日: 2024-03-20)
主引用文献Wang, J.,Li, J.,Zhao, H.,Sheng, G.,Wang, M.,Yin, M.,Wang, Y.
Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
Cell, 163:840-853, 2015
Cited by
PubMed Abstract: Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR array. We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion into the CRISPR locus. Our study reveals a protospacer DNA comprising a 23-bp duplex bracketed by tyrosine residues, together with anchored flanking 3' overhang segments. The PAM-complementary sequence in the 3' overhang is recognized by the Cas1a catalytic subunits in a base-specific manner, and subsequent cleavage at positions 5 nt from the duplex boundary generates a 33-nt DNA intermediate that is incorporated into the CRISPR array via a cut-and-paste mechanism. Upon protospacer binding, Cas1-Cas2 undergoes a significant conformational change, generating a flat surface conducive to proper protospacer recognition. Here, our study provides important structure-based mechanistic insights into PAM-dependent spacer acquisition.
PubMed: 26478180
DOI: 10.1016/j.cell.2015.10.008
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.6 Å)
構造検証レポート
Validation report summary of 5dlj
検証レポート(詳細版)ダウンロードをダウンロード

239149

件を2025-07-23に公開中

PDB statisticsPDBj update infoContact PDBjnumon