5DJU

Crystal structure of LOV2 (C450A) domain in complex with Zdk3

5DJU の概要

• エントリーDOI: 10.2210/pdb5dju/pdb
• 分子名称: Engineered protein, Zdk3 affibody, NPH1-2, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• 機能のキーワード: protein binding, protein engineering, signaling protein, photoswitch, light-induced signal transduction, lov2, affibody, complex
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48000.72

構造登録者

Tarnawski, M.,Wang, H.,Yumerefendi, H.,Hahn, K.M.,Schlichting, I. (登録日: 2015-09-02, 公開日: 2016-07-20, 最終更新日: 2024-01-10)

主引用文献

Wang, H.,Vilela, M.,Winkler, A.,Tarnawski, M.,Schlichting, I.,Yumerefendi, H.,Kuhlman, B.,Liu, R.,Danuser, G.,Hahn, K.M.
LOVTRAP: an optogenetic system for photoinduced protein dissociation.
Nat.Methods, 13:755-758, 2016

PubMed Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

PubMed: 27427858
DOI: 10.1038/nmeth.3926

実験手法

X-RAY DIFFRACTION (2.1 Å)