5DA5

Crystal structure of Rhodospirillum rubrum Rru_A0973

5DA5 の概要

• エントリーDOI: 10.2210/pdb5da5/pdb
• 分子名称: Rru_A0973, FE (III) ION, GLYCOLIC ACID, ... (5 entities in total)
• 機能のキーワード: ferritin, iron, ferroxidase, oxidoreductase
• 由来する生物種: Rhodospirillum rubrum
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 404444.60

構造登録者

He, D.,Vanden Hehier, S.,Georgiev, A.,Altenbach, K.,Tarrant, E.,Mackay, C.L.,Waldron, K.J.,Clarke, D.J.,Marles-Wright, J. (登録日: 2015-08-19, 公開日: 2016-08-10, 最終更新日: 2024-01-10)

主引用文献

He, D.,Hughes, S.,Vanden-Hehir, S.,Georgiev, A.,Altenbach, K.,Tarrant, E.J.,Mackay, C.L.,Waldron, K.J.,Clarke, D.J.,Marles-Wright, J.
Structural characterization of encapsulated ferritin provides insight into iron storage in bacterial nanocompartments.
Elife, 5:-, 2016

PubMed Abstract: Ferritins are ubiquitous proteins that oxidise and store iron within a protein shell to protect cells from oxidative damage. We have characterized the structure and function of a new member of the ferritin superfamily that is sequestered within an encapsulin capsid. We show that this encapsulated ferritin (EncFtn) has two main alpha helices, which assemble in a metal dependent manner to form a ferroxidase center at a dimer interface. EncFtn adopts an open decameric structure that is topologically distinct from other ferritins. While EncFtn acts as a ferroxidase, it cannot mineralize iron. Conversely, the encapsulin shell associates with iron, but is not enzymatically active, and we demonstrate that EncFtn must be housed within the encapsulin for iron storage. This encapsulin nanocompartment is widely distributed in bacteria and archaea and represents a distinct class of iron storage system, where the oxidation and mineralization of iron are distributed between two proteins.

PubMed: 27529188
DOI: 10.7554/eLife.18972

実験手法

X-RAY DIFFRACTION (2.064 Å)