5D6B
Human fumarase (wild type)
5D6B の概要
| エントリーDOI | 10.2210/pdb5d6b/pdb |
| 分子名称 | Fumarate hydratase, mitochondrial (2 entities in total) |
| 機能のキーワード | lyase, tca cycle, fumarate hydratase |
| 由来する生物種 | Homo sapiens (Human) |
| タンパク質・核酸の鎖数 | 1 |
| 化学式量合計 | 49739.01 |
| 構造登録者 | |
| 主引用文献 | Pereira de Padua, R.A.,Nonato, M.C. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of recombinant human fumarase. Acta Crystallogr F Struct Biol Commun, 70:120-122, 2014 Cited by PubMed Abstract: Human fumarase (HsFH) is a well-known citric acid cycle enzyme and is therefore a key component in energy metabolism. Genetic studies on human patients have shown that polymorphisms in the fumarase gene are responsible for diseases such as hereditary leiomyomatosis and renal cell cancer. As a first step in unravelling the molecular basis of the mechanism of fumarase deficiency in genetic disorders, the HsFH gene was cloned in pET-28a, heterologously expressed in Escherichia coli, purified by nickel-affinity chromatography and crystallized using the vapour-diffusion technique. X-ray diffraction experiments were performed at a synchrotron source and the structure was solved at 2.1 Å resolution by molecular replacement. PubMed: 24419633DOI: 10.1107/S2053230X13033955 主引用文献が同じPDBエントリー |
| 実験手法 | X-RAY DIFFRACTION (2.1 Å) |
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