5D51

Krypton derivatization of an O2-tolerant membrane-bound [NiFe] hydrogenase reveals a hydrophobic gas tunnel network

Summary for 5D51

• Entry DOI: 10.2210/pdb5d51/pdb
• Descriptor: Uptake hydrogenase large subunit, Uptake hydrogenase small subunit, NI-FE OXIDIZED ACTIVE CENTER, ... (10 entities in total)
• Functional Keywords: oxidoreductase, [nife] hydrogenase, knallgasbacteria, proteobacteria, aerobic hydrogen bacteria, dehydrogenase, hydrogen, dihydrogen, hydrogen catalysis, metalloenzyme, metalloprotein catalytic center, nickel-iron cofactor, bimetallic, ni-fe active site, iron-sulfur cluster, t-cluster, [4fe-3s] cluster, [3fe-4s] cluster, [4fe-4s] cluster, oxidized state, oxygen-tolerant hydrogenase, membrane-bound, krypton, noble gas derivatization, hydrophobic tunnel, gas transport
• Biological source: Cupriavidus necator (Ralstonia eutropha); More
• Cellular location: Cell membrane; Peripheral membrane protein: P31891 P31892
• Total number of polymer chains: 2
• Total formula weight: 107496.68

Authors

Kalms, J.,Schmidt, A.,Frielingsdorf, S.,van der Linden, P.,von Stetten, D.,Lenz, O.,Carpentier, P.,Scheerer, P. (deposition date: 2015-08-10, release date: 2016-03-23, Last modification date: 2024-01-10)

• Primary citation: Kalms, J.,Schmidt, A.,Frielingsdorf, S.,van der Linden, P.,von Stetten, D.,Lenz, O.,Carpentier, P.,Scheerer, P.; Krypton Derivatization of an O2 -Tolerant Membrane-Bound [NiFe] Hydrogenase Reveals a Hydrophobic Tunnel Network for Gas Transport.; Angew.Chem.Int.Ed.Engl., 55:5586-5590, 2016; PubMed: 26913499; DOI: 10.1002/anie.201508976

Experimental method

X-RAY DIFFRACTION (1.47 Å)