5D43

crystal structrue of Mouse centrin 1 in calcium-saturated form

Summary for 5D43

• Entry DOI: 10.2210/pdb5d43/pdb
• Descriptor: Centrin-1, CALCIUM ION (3 entities in total)
• Functional Keywords: calcium-binding protein, centrin, ef-hand motif, metal binding protein
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 2
• Total formula weight: 41430.92

Authors

Park, J.H.,Kim, S.Y.,Kim, D.S. (deposition date: 2015-08-07, release date: 2016-09-07, Last modification date: 2023-11-08)

Primary citation

Kim, S.Y.,Kim, D.S.,Hong, J.E.,Park, J.H.
Crystal Structure of Wild-Type Centrin 1 from Mus musculus Occupied by Ca2.
Biochemistry Mosc., 82:1129-1139, 2017

PubMed Abstract: Mus musculus centrin 1 (MmCen1) is located at the cilium of photoreceptor cells connecting the outer segment through signal transduction components to the metabolically active inner segment. In the cilium, MmCen1 is involved in the translocation of transducin between compartments as a result of photoreceptor activation. In this study, we report the crystal structure of wild-type MmCen1 and its Ca2+-binding properties using structure-based mutagenesis. The crystal structure exhibits three structural features, i.e. four Ca2+ equally occupied at each EF-hand motif, structural changes accompanying helix motion at the N- and C-lobes, and adoption of N-C type dimerization when Ca2+ binds to EF-hand I and II in the N-lobe. The presence of MmCen1 dimers was confirmed in solution by native PAGE. Isothermal titration calorimetry data showed sequential binding of Ca2+ at four independent sites. Mutations S45A and D49A in EF-hand I alone disrupted the Ca2+-binding property of the wild-type protein. Based on the crystal structure of MmCen1, we suggest that a dimerization mode between the N- and C-lobes may be required by Ca2+ binding at the N-lobe.

PubMed: 29037133
DOI: 10.1134/S0006297917100054

Experimental method

X-RAY DIFFRACTION (2.82 Å)