5D2C

Reaction of phosphorylated CheY with imidazole 1 of 3

5D2C の概要

• エントリーDOI: 10.2210/pdb5d2c/pdb
• 関連するPDBエントリー: 3FFW
• 分子名称: Chemotaxis protein CheY, MANGANESE (II) ION, BERYLLIUM TRIFLUORIDE ION, ... (7 entities in total)
• 機能のキーワード: two component signaling, asp to his phosphotransfer, response regulator, receiver domain, metal binding protein
• 由来する生物種: Escherichia coli O157:H7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29166.31

構造登録者

Page, S.,Silversmith, R.E.,Bourret, R.B.,Collins, E.J. (登録日: 2015-08-05, 公開日: 2016-03-09, 最終更新日: 2024-03-06)

主引用文献

Page, S.C.,Silversmith, R.E.,Collins, E.J.,Bourret, R.B.
Imidazole as a Small Molecule Analogue in Two-Component Signal Transduction.
Biochemistry, 54:7248-7260, 2015

PubMed Abstract: In two-component signal transduction systems (TCSs), responses to stimuli are mediated through phosphotransfer between protein components. Canonical TCSs use His → Asp phosphotransfer in which phosphoryl groups are transferred from a conserved His on a sensory histidine kinase (HK) to a conserved Asp on a response regulator (RR). RRs contain the catalytic core of His → Asp phosphotransfer, evidenced by the ability of RRs to autophosphorylate with small molecule analogues of phospho-His proteins. Phosphorelays are a more complex variation of TCSs that additionally utilize Asp → His phosphotransfer through the use of an additional component, the histidine-containing phosphotransfer domain (Hpt), which reacts with RRs both as phosphodonors and phosphoacceptors. Here we show that imidazole has features of a rudimentary Hpt. Imidazole acted as a nucleophile and attacked phosphorylated RRs (RR-P) to produce monophosphoimidazole (MPI) and unphosphorylated RR. Phosphotransfer from RR-P to imidazole required the intact RR active site, indicating that the RR provided the core catalytic machinery for Asp → His phosphotransfer. Imidazole functioned in an artificial phosphorelay to transfer phosphoryl groups between unrelated RRs. The X-ray crystal structure of an activated RR·imidazole complex showed imidazole oriented in the RR active site similarly to the His of an Hpt. Imidazole interacted with RR nonconserved active site residues, which influenced the relative reactivity of RR-P with imidazole versus water. Rate constants for reaction of imidazole or MPI with chimeric RRs suggested that the RR active site contributes to the kinetic preferences exhibited by the YPD1 Hpt.

PubMed: 26569142
DOI: 10.1021/acs.biochem.5b01082

実験手法

X-RAY DIFFRACTION (2.06 Å)