5D1I

Structure of Cyclic nucleotide-binding-like protein from Brucella abortus bv. 1 str. 9-941

5D1I の概要

• エントリーDOI: 10.2210/pdb5d1i/pdb
• 分子名称: Cyclic nucleotide-binding protein (2 entities in total)
• 機能のキーワード: beta barrel, unknown function
• 由来する生物種: Brucella abortus biovar 1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28910.42

構造登録者

He, Z.,Dong, J.,Li, X.,Gao, Y. (登録日: 2015-08-04, 公開日: 2015-09-09, 最終更新日: 2024-10-16)

主引用文献

He, Z.,Gao, Y.,Dong, J.,Ke, Y.,Li, X.,Chen, Z.,Zhang, X.C.
Crystal structure of cyclic nucleotide-binding-like protein from Brucella abortus
Biochem.Biophys.Res.Commun., 468:647-652, 2015

PubMed Abstract: The cyclic nucleotide-binding (CNB)-like protein (CNB-L) from Brucella abortus shares sequence homology with CNB domain-containing proteins. We determined the crystal structure of CNB-L at 2.0 Å resolution in the absence of its C-terminal helix and nucleotide. The 3D structure of CNB-L is in a two-fold symmetric form. Each protomer shows high structure similarity to that of cGMP-binding domain-containing proteins, and likely mimics their nucleotide-free conformation. A key residue, Glu17, mediates the dimerization and prevents binding of cNMP to the canonical ligand-pocket. The structurally observed dimer of CNB-L is stable in solution, and thus is likely to be biologically relevant.

PubMed: 26549229
DOI: 10.1016/j.bbrc.2015.11.005

実験手法

X-RAY DIFFRACTION (2 Å)