5CW9

Crystal structure of De novo designed ferredoxin-ferredoxin domain insertion protein

5CW9 の概要

• エントリーDOI: 10.2210/pdb5cw9/pdb
• 関連するPDBエントリー: 4KY3 4KYZ
• 分子名称: De novo designed ferredoxin-ferredoxin domain insertion protein (1 entity in total)
• 機能のキーワード: designed, de novo, ferredoxin, domain insertion, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16838.68

構造登録者

DiMaio, F.,King, I.C.,Gleixner, J.,Doyle, L.,Stoddard, B.,Baker, D. (登録日: 2015-07-28, 公開日: 2015-09-23, 最終更新日: 2024-03-06)

主引用文献

King, I.C.,Gleixner, J.,Doyle, L.,Kuzin, A.,Hunt, J.F.,Xiao, R.,Montelione, G.T.,Stoddard, B.L.,DiMaio, F.,Baker, D.
Precise assembly of complex beta sheet topologies from de novo designed building blocks.
Elife, 4:-, 2015

PubMed Abstract: Design of complex alpha-beta protein topologies poses a challenge because of the large number of alternative packing arrangements. A similar challenge presumably limited the emergence of large and complex protein topologies in evolution. Here, we demonstrate that protein topologies with six and seven-stranded beta sheets can be designed by insertion of one de novo designed beta sheet containing protein into another such that the two beta sheets are merged to form a single extended sheet, followed by amino acid sequence optimization at the newly formed strand-strand, strand-helix, and helix-helix interfaces. Crystal structures of two such designs closely match the computational design models. Searches for similar structures in the SCOP protein domain database yield only weak matches with different beta sheet connectivities. A similar beta sheet fusion mechanism may have contributed to the emergence of complex beta sheets during natural protein evolution.

PubMed: 26650357
DOI: 10.7554/eLife.11012

実験手法

X-RAY DIFFRACTION (3.108 Å)