5CW3

Structure of CfBRCC36-CfKIAA0157 complex (Zn Edge)

Summary for 5CW3

• Entry DOI: 10.2210/pdb5cw3/pdb
• Related: 5CW4 5CW5 5CW6
• Descriptor: BRCA1/BRCA2-containing complex subunit 3, Protein FAM175B, ZINC ION, ... (4 entities in total)
• Functional Keywords: metal dependent enzyme, metal binding protein
• Biological source: Camponotus floridanus (Florida carpenter ant); More
• Total number of polymer chains: 4
• Total formula weight: 123079.94

Authors

Zeqiraj, E. (deposition date: 2015-07-27, release date: 2015-09-16, Last modification date: 2024-03-06)

Primary citation

Zeqiraj, E.,Tian, L.,Piggott, C.A.,Pillon, M.C.,Duffy, N.M.,Ceccarelli, D.F.,Keszei, A.F.,Lorenzen, K.,Kurinov, I.,Orlicky, S.,Gish, G.D.,Heck, A.J.,Guarne, A.,Greenberg, R.A.,Sicheri, F.
Higher-Order Assembly of BRCC36-KIAA0157 Is Required for DUB Activity and Biological Function.
Mol.Cell, 59:970-983, 2015

PubMed Abstract: BRCC36 is a Zn(2+)-dependent deubiquitinating enzyme (DUB) that hydrolyzes lysine-63-linked ubiquitin chains as part of distinct macromolecular complexes that participate in either interferon signaling or DNA-damage recognition. The MPN(+) domain protein BRCC36 associates with pseudo DUB MPN(-) proteins KIAA0157 or Abraxas, which are essential for BRCC36 enzymatic activity. To understand the basis for BRCC36 regulation, we have solved the structure of an active BRCC36-KIAA0157 heterodimer and an inactive BRCC36 homodimer. Structural and functional characterizations show how BRCC36 is switched to an active conformation by contacts with KIAA0157. Higher-order association of BRCC36 and KIAA0157 into a dimer of heterodimers (super dimers) was required for DUB activity and interaction with targeting proteins SHMT2 and RAP80. These data provide an explanation of how an inactive pseudo DUB allosterically activates a cognate DUB partner and implicates super dimerization as a new regulatory mechanism underlying BRCC36 DUB activity, subcellular localization, and biological function.

PubMed: 26344097
DOI: 10.1016/j.molcel.2015.07.028

Experimental method

X-RAY DIFFRACTION (2.55 Å)