5CW0
Investigation of RNA structure in satellite panicum mosaic virus
Summary for 5CW0
| Entry DOI | 10.2210/pdb5cw0/pdb |
| Related | 1STM |
| Descriptor | Coat protein (1 entity in total) |
| Functional Keywords | capsid protein, satellite virus, virus |
| Biological source | Satellite panicum mosaic virus (SPMV) |
| Total number of polymer chains | 1 |
| Total formula weight | 16983.16 |
| Authors | Makino, D.L.,Day, J.,Larson, S.B.,McPherson, A. (deposition date: 2015-07-27, release date: 2017-10-11, Last modification date: 2023-09-27) |
| Primary citation | Makino, D.L.,Day, J.,Larson, S.B.,McPherson, A. Investigation of RNA structure in satellite panicum mosaic virus. Virology, 351:420-431, 2006 Cited by PubMed Abstract: Three new crystal forms of satellite panicum mosaic virus (SPMV) were grown and their structures solved from X-ray diffraction data using molecular replacement techniques. The crystals were grown under conditions of pH and ionic strength that were appreciably different then those used for the original structure determination. In rhombohedral crystals grown at pH 8.5 and low ionic strength PEG 3350 solutions, Fourier syntheses revealed segments, ten amino acid residues long, of amino-terminal polypeptides not previously seen, as well as masses of electron density within concavities on the interior of the capsid, which appeared in the neighborhoods of icosahedral five- and threefold axes. The densities were compatible with secondary structural domains of RNA, and they included a segment of double helical RNA of about four to five base pairs oriented, at least approximately, along the fivefold axes. The distribution of RNA observed for SPMV appears to be distinctly different than the encapsidated nucleic acid conformation previously suggested for another satellite virus, satellite tobacco mosaic virus. This study further shows that analysis of viruses in crystals grown under different chemical conditions may reveal additional information regarding the structure of encapsidated RNA. PubMed: 16677679DOI: 10.1016/j.virol.2006.03.028 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (4.6 Å) |
Structure validation
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