5CUP

Structure of Rhodopseudomonas palustris PduL - phosphate bound form

Summary for 5CUP

• Entry DOI: 10.2210/pdb5cup/pdb
• Related: 5CUO
• Descriptor: Phosphate propanoyltransferase, ZINC ION, PHOSPHATE ION, ... (4 entities in total)
• Functional Keywords: enzyme, transferase
• Biological source: Rhodopseudomonas palustris (strain BisB18)
• Total number of polymer chains: 2
• Total formula weight: 44362.54

Authors

Sutter, M.,Erbilgin, O.,Kerfeld, C.A. (deposition date: 2015-07-24, release date: 2016-03-23, Last modification date: 2024-03-06)

Primary citation

Erbilgin, O.,Sutter, M.,Kerfeld, C.A.
The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle.
Plos Biol., 14:e1002399-e1002399, 2016

PubMed Abstract: Bacterial Microcompartments (BMCs) are proteinaceous organelles that encapsulate critical segments of autotrophic and heterotrophic metabolic pathways; they are functionally diverse and are found across 23 different phyla. The majority of catabolic BMCs (metabolosomes) compartmentalize a common core of enzymes to metabolize compounds via a toxic and/or volatile aldehyde intermediate. The core enzyme phosphotransacylase (PTAC) recycles Coenzyme A and generates an acyl phosphate that can serve as an energy source. The PTAC predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). Here, we report two high-resolution PduL crystal structures with bound substrates. The PduL fold is unrelated to that of Pta; it contains a dimetal active site involved in a catalytic mechanism distinct from that of the housekeeping PTAC. Accordingly, PduL and Pta exemplify functional, but not structural, convergent evolution. The PduL structure, in the context of the catalytic core, completes our understanding of the structural basis of cofactor recycling in the metabolosome lumen.

PubMed: 26959993
DOI: 10.1371/journal.pbio.1002399

Experimental method

X-RAY DIFFRACTION (2.1 Å)