5CUK

Crystal structure of the PscP SS domain

5CUK の概要

• エントリーDOI: 10.2210/pdb5cuk/pdb
• 関連するPDBエントリー: 5CUL
• 分子名称: Ruler protein, GLYCEROL (3 entities in total)
• 機能のキーワード: molecular ruler secretion system, cell invasion
• 由来する生物種: Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13561.22

構造登録者

Bergeron, J.R.C.,Strynadka, N.C.J. (登録日: 2015-07-24, 公開日: 2015-12-02, 最終更新日: 2024-03-06)

主引用文献

Bergeron, J.R.,Fernandez, L.,Wasney, G.A.,Vuckovic, M.,Reffuveille, F.,Hancock, R.E.,Strynadka, N.C.
The Structure of a Type 3 Secretion System (T3SS) Ruler Protein Suggests a Molecular Mechanism for Needle Length Sensing.
J.Biol.Chem., 291:1676-1691, 2016

PubMed Abstract: The type 3 secretion system (T3SS) and the bacterial flagellum are related pathogenicity-associated appendages found at the surface of many disease-causing bacteria. These appendages consist of long tubular structures that protrude away from the bacterial surface to interact with the host cell and/or promote motility. A proposed "ruler" protein tightly regulates the length of both the T3SS and the flagellum, but the molecular basis for this length control has remained poorly characterized and controversial. Using the Pseudomonas aeruginosa T3SS as a model system, we report the first structure of a T3SS ruler protein, revealing a "ball-and-chain" architecture, with a globular C-terminal domain (the ball) preceded by a long intrinsically disordered N-terminal polypeptide chain. The dimensions and stability of the globular domain do not support its potential passage through the inner lumen of the T3SS needle. We further demonstrate that a conserved motif at the N terminus of the ruler protein interacts with the T3SS autoprotease in the cytosolic side. Collectively, these data suggest a potential mechanism for needle length sensing by ruler proteins, whereby upon T3SS needle assembly, the ruler protein's N-terminal end is anchored on the cytosolic side, with the globular domain located on the extracellular end of the growing needle. Sequence analysis of T3SS and flagellar ruler proteins shows that this mechanism is probably conserved across systems.

PubMed: 26589798
DOI: 10.1074/jbc.M115.684423

実験手法

X-RAY DIFFRACTION (2.1 Å)