5CR4

Crystal structure of the Sleeping Beauty transposase catalytic domain

5CR4 の概要

• エントリーDOI: 10.2210/pdb5cr4/pdb
• 分子名称: Sleeping Beauty transposase, SB100X, SULFATE ION, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: transposase, tc1/mariner family, rnaseh fold, hydrolase
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57174.98

構造登録者

Voigt, F.,Barabas, O. (登録日: 2015-07-22, 公開日: 2016-03-30, 最終更新日: 2024-01-10)

主引用文献

Voigt, F.,Wiedemann, L.,Zuliani, C.,Querques, I.,Sebe, A.,Mates, L.,Izsvak, Z.,Ivics, Z.,Barabas, O.
Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.
Nat Commun, 7:11126-11126, 2016

PubMed Abstract: Sleeping Beauty (SB) is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms. It is mobilized by a transposase enzyme that catalyses DNA cleavage and integration at short specific sequences at the transposon ends. To facilitate SB's applications, here we determine the crystal structure of the transposase catalytic domain and use it to model the SB transposase/transposon end/target DNA complex. Together with biochemical and cell-based transposition assays, our structure reveals mechanistic insights into SB transposition and rationalizes previous hyperactive transposase mutations. Moreover, our data enables us to design two additional hyperactive transposase variants. Our work provides a useful resource and proof-of-concept for structure-based engineering of tailored SB transposases.

PubMed: 27025571
DOI: 10.1038/ncomms11126

実験手法

X-RAY DIFFRACTION (1.4 Å)