5CPI

Nucleosome containing unmethylated Sat2R DNA

5CPI の概要

• エントリーDOI: 10.2210/pdb5cpi/pdb
• 関連するPDBエントリー: 5CPJ 5CPK
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: histone fold, dna binding, nucleus, nucleosome, chromatin formation, dna methylation, structural protein-dna complex, structural protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 202224.75

構造登録者

Osakabe, A.,Arimura, Y.,Adachi, F.,Maehara, K.,Ohkawa, Y.,Kurumizaka, H. (登録日: 2015-07-21, 公開日: 2015-10-28, 最終更新日: 2023-11-08)

主引用文献

Osakabe, A.,Adachi, F.,Arimura, Y.,Maehara, K.,Ohkawa, Y.,Kurumizaka, H.
Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA.
Open Biology, 5:-, 2015

PubMed Abstract: DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reportedly hypomethylated in cancer cells, suggesting that the methylation status may affect the chromatin structure around the pericentric regions in tumours. In this study, we mapped the nucleosome positioning on the satellite 2 sequence in vitro and found that DNA methylation modestly affects the distribution of the nucleosome positioning. The micrococcal nuclease assay revealed that the DNA end flexibility of the nucleosomes changes, depending on the DNA methylation status. However, the structures and thermal stabilities of the nucleosomes are unaffected by DNA methylation. These findings provide new information to understand how DNA methylation functions in regulating pericentric heterochromatin formation and maintenance in normal and malignant cells.

PubMed: 26446621
DOI: 10.1098/rsob.150128

実験手法

X-RAY DIFFRACTION (2.902 Å)