5CO8

Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion

5CO8 の概要

• エントリーDOI: 10.2210/pdb5co8/pdb
• 分子名称: DNA (31-MER), Nuclease-like protein, DNA (5'-D(*AP*GP*AP*CP*TP*GP*CP*AP*GP*TP*TP*GP*AP*GP*TP*C)-3'), ... (7 entities in total)
• 機能のキーワード: holiday junction, resolvase, complex, dna, hydrolase
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 122975.32

構造登録者

Liu, Y.J.,Freeman, A.D.J.,Declais, A.C.,Wilson, T.J.,Gartner, A.,Lilley, D.M.J. (登録日: 2015-07-20, 公開日: 2016-01-13, 最終更新日: 2024-10-23)

主引用文献

Liu, Y.,Freeman, A.D.,Declais, A.C.,Wilson, T.J.,Gartner, A.,Lilley, D.M.
Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA.
Cell Rep, 13:2565-2575, 2015

PubMed Abstract: We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 Å resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a monomer of active GEN1 bound to the product of resolution cleavage, with an extensive DNA binding interface for both helical arms. Within the crystal lattice, a GEN1 dimer interface juxtaposes two products, whereby they can be reconnected into a four-way junction, the structure of which agrees with that determined in solution. The reconnection requires some opening of the DNA structure at the center, in agreement with permanganate probing and 2-aminopurine fluorescence. The structure shows that a relaxation of the DNA structure accompanies cleavage, suggesting how second-strand cleavage is accelerated to ensure productive resolution of the junction.

PubMed: 26686639
DOI: 10.1016/j.celrep.2015.11.042

実験手法

X-RAY DIFFRACTION (2.4 Å)