5CK7

Mouse ADP-dependent Glucokinase; AMP bound

5CK7 の概要

• エントリーDOI: 10.2210/pdb5ck7/pdb
• 関連するPDBエントリー: 5CCF
• 分子名称: ADP-dependent glucokinase, ADENOSINE MONOPHOSPHATE (3 entities in total)
• 機能のキーワード: adpgk, ribokinase, kinase, amp, transferase
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53414.96

構造登録者

Richter, J.P.,Ronimus, R.S.,Sutherland-Smith, A.J. (登録日: 2015-07-15, 公開日: 2015-11-18, 最終更新日: 2024-10-16)

主引用文献

Richter, J.P.,Goroncy, A.K.,Ronimus, R.S.,Sutherland-Smith, A.J.
The Structural and Functional Characterization of Mammalian ADP-dependent Glucokinase.
J.Biol.Chem., 291:3694-3704, 2016

PubMed Abstract: The enzyme-catalyzed phosphorylation of glucose to glucose-6-phosphate is a reaction central to the metabolism of all life. ADP-dependent glucokinase (ADPGK) catalyzes glucose-6-phosphate production, utilizing ADP as a phosphoryl donor in contrast to the more well characterized ATP-requiring hexokinases. ADPGK is found in Archaea and metazoa; in Archaea, ADPGK participates in a glycolytic role, but a function in most eukaryotic cell types remains unknown. We have determined structures of the eukaryotic ADPGK revealing a ribokinase-like tertiary fold similar to archaeal orthologues but with significant differences in some secondary structural elements. Both the unliganded and the AMP-bound ADPGK structures are in the "open" conformation. The structures reveal the presence of a disulfide bond between conserved cysteines that is positioned at the nucleotide-binding loop of eukaryotic ADPGK. The AMP-bound ADPGK structure defines the nucleotide-binding site with one of the disulfide bond cysteines coordinating the AMP with its main chain atoms, a nucleotide-binding motif that appears unique to eukaryotic ADPGKs. Key amino acids at the active site are structurally conserved between mammalian and archaeal ADPGK, and site-directed mutagenesis has confirmed residues essential for enzymatic activity. ADPGK is substrate inhibited by high glucose concentration and shows high specificity for glucose, with no activity for other sugars, as determined by NMR spectroscopy, including 2-deoxyglucose, the glucose analogue used for tumor detection by positron emission tomography.

PubMed: 26555263
DOI: 10.1074/jbc.M115.679902

実験手法

X-RAY DIFFRACTION (2.99 Å)