5CIS

The CUB1-EGF-CUB2 domains of rat MBL-associated serine protease-2 (MASP-2) bound to Ca2+

5CIS の概要

• エントリーDOI: 10.2210/pdb5cis/pdb
• 分子名称: Mannan-binding lectin serine peptidase 2, CALCIUM ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: masp, cub1-egf-cub2, complement activation, lectin pathway, hydrolase
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31868.42

構造登録者

Nan, R.,Furze, C.M.,Wright, D.W.,Gor, J.,Wallis, R.,Perkins, S.J. (登録日: 2015-07-13, 公開日: 2017-01-18, 最終更新日: 2024-10-23)

主引用文献

Nan, R.,Furze, C.M.,Wright, D.W.,Gor, J.,Wallis, R.,Perkins, S.J.
Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.
Structure, 25:364-375, 2017

PubMed Abstract: The lectin pathway of complement is activated by complexes comprising a recognition component (mannose-binding lectin, serum ficolins, collectin-LK or collectin-K1) and a serine protease (MASP-1 or MASP-2). MASP-1 activates MASP-2, and MASP-2 cleaves C4 and C4b-bound C2. To clarify activation, new crystal structures of Ca-bound MASP dimers were determined, together with their solution structures from X-ray scattering, analytical ultracentrifugation, and atomistic modeling. Solution structures of the CUB1-EGF-CUB2 dimer of each MASP indicate that the two CUB2 domains were tilted by as much as 90° compared with the crystal structures, indicating considerable flexibility at the EGF-CUB2 junction. Solution structures of the full-length MASP dimers in their zymogen and activated forms revealed similar structures that were much more bent than anticipated from crystal structures. We conclude that MASP-1 and MASP-2 are flexible at multiple sites and that this flexibility may permit both intra- and inter-complex activation.

PubMed: 28111019
DOI: 10.1016/j.str.2016.12.014

実験手法

X-RAY DIFFRACTION (2.58 Å)