5CBK

Crystal structure of the strigolactone receptor ShHTL5 from Striga hermonthica

5CBK の概要

• エントリーDOI: 10.2210/pdb5cbk/pdb
• 分子名称: ShHTL5, MAGNESIUM ION, 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL, ... (5 entities in total)
• 機能のキーワード: striga hermonthica, strigolactone, signalling, receptor, shhtl5, alpha/beta hydrolase, signaling protein
• 由来する生物種: Striga hermonthica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31320.28

構造登録者

Stogios, P.J.,Onopriyenko, O.,Yim, V.,Savchenko, A. (登録日: 2015-07-01, 公開日: 2015-10-21, 最終更新日: 2023-09-27)

主引用文献

Toh, S.,Holbrook-Smith, D.,Stogios, P.J.,Onopriyenko, O.,Lumba, S.,Tsuchiya, Y.,Savchenko, A.,McCourt, P.
Structure-function analysis identifies highly sensitive strigolactone receptors in Striga.
Science, 350:203-207, 2015

PubMed Abstract: Strigolactones are naturally occurring signaling molecules that affect plant development, fungi-plant interactions, and parasitic plant infestations. We characterized the function of 11 strigolactone receptors from the parasitic plant Striga hermonthica using chemical and structural biology. We found a clade of polyspecific receptors, including one that is sensitive to picomolar concentrations of strigolactone. A crystal structure of a highly sensitive strigolactone receptor from Striga revealed a larger binding pocket than that of the Arabidopsis receptor, which could explain the increased range of strigolactone sensitivity. Thus, the sensitivity of Striga to strigolactones from host plants is driven by receptor sensitivity. By expressing strigolactone receptors in Arabidopsis, we developed a bioassay that can be used to identify chemicals and crops with altered strigolactone levels.

PubMed: 26450211
DOI: 10.1126/science.aac9476

実験手法

X-RAY DIFFRACTION (2.462 Å)