5C8J

A YidC-like protein in the archaeal plasma membrane

5C8J の概要

• エントリーDOI: 10.2210/pdb5c8j/pdb
• 分子名称: Antibody fragment, heavy chain, Antibody fragment, light chain, Protein MJ0480, ... (4 entities in total)
• 機能のキーワード: membrane protein insertase, membrane protein
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 292699.79

構造登録者

Borowska, M.T.,Dominik, P.K.,Anghel, S.A.,Kossiakoff, A.A.,Keenan, R.J. (登録日: 2015-06-25, 公開日: 2015-09-02, 最終更新日: 2024-10-09)

主引用文献

Borowska, M.T.,Dominik, P.K.,Anghel, S.A.,Kossiakoff, A.A.,Keenan, R.J.
A YidC-like Protein in the Archaeal Plasma Membrane.
Structure, 23:1715-1724, 2015

PubMed Abstract: Cells possess specialized machinery to direct the insertion of membrane proteins into the lipid bilayer. In bacteria, the essential protein YidC inserts certain proteins into the plasma membrane, and eukaryotic orthologs are present in the mitochondrial inner membrane and the chloroplast thylakoid membrane. The existence of homologous insertases in archaea has been proposed based on phylogenetic analysis. However, limited sequence identity, distinct architecture, and the absence of experimental data have made this assignment ambiguous. Here we describe the 3.5-Å crystal structure of an archaeal DUF106 protein from Methanocaldococcus jannaschii (Mj0480), revealing a lipid-exposed hydrophilic surface presented by a conserved YidC-like fold. Functional analysis reveals selective binding of Mj0480 to ribosomes displaying a stalled YidC substrate, and a direct interaction between the buried hydrophilic surface of Mj0480 and the nascent chain. These data provide direct experimental evidence that the archaeal DUF106 proteins are YidC/Oxa1/Alb3-like insertases of the archaeal plasma membrane.

PubMed: 26256539
DOI: 10.1016/j.str.2015.06.025

実験手法

X-RAY DIFFRACTION (3.502 Å)