5C7W

5'-monophosphate Z:P Guanine Riboswitch bound to hypoxanthine.

5C7W の概要

• エントリーDOI: 10.2210/pdb5c7w/pdb
• 分子名称: 5'-monophosphate Z:P guanine riboswitch, COBALT HEXAMMINE(III), HYPOXANTHINE (3 entities in total)
• 機能のキーワード: expanded rna; riboswitch; unnatural base pair, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22975.83

構造登録者

Hernandez, A.R.,Shao, Y.,Hoshika, S.,Yang, Z.,Shelke, S.A.,Herrou, J.,Kim, H.-J.,Kim, M.-J.,Piccirilli, J.A.,Benner, S.A. (登録日: 2015-06-25, 公開日: 2015-08-12, 最終更新日: 2024-05-01)

主引用文献

Hernandez, A.R.,Shao, Y.,Hoshika, S.,Yang, Z.,Shelke, S.A.,Herrou, J.,Kim, H.J.,Kim, M.J.,Piccirilli, J.A.,Benner, S.A.
A Crystal Structure of a Functional RNA Molecule Containing an Artificial Nucleobase Pair.
Angew.Chem.Int.Ed.Engl., 54:9853-9856, 2015

PubMed Abstract: As one of its goals, synthetic biology seeks to increase the number of building blocks in nucleic acids. While efforts towards this goal are well advanced for DNA, they have hardly begun for RNA. Herein, we present a crystal structure for an RNA riboswitch where a stem C:G pair has been replaced by a pair between two components of an artificially expanded genetic-information system (AEGIS), Z and P, (6-amino-5-nitro-2(1H)-pyridone and 2-amino-imidazo[1,2-a]-1,3,5-triazin-4-(8H)-one). The structure shows that the Z:P pair does not greatly change the conformation of the RNA molecule nor the details of its interaction with a hypoxanthine ligand. This was confirmed in solution by in-line probing, which also measured a 3.7 nM affinity of the riboswitch for guanine. These data show that the Z:P pair mimics the natural Watson-Crick geometry in RNA in the first example of a crystal structure of an RNA molecule that contains an orthogonal added nucleobase pair.

PubMed: 26223188
DOI: 10.1002/anie.201504731

実験手法

X-RAY DIFFRACTION (3.22 Å)