5C02

Influenza A M2 transmembrane domain drug-resistant S31N mutant at pH 8.0

5C02 の概要

• エントリーDOI: 10.2210/pdb5c02/pdb
• 分子名称: Matrix protein 2, STRONTIUM ION, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: membrane protein, influenza, drug resistance
• 由来する生物種: Influenza A virus (A/Udorn/307/1972(H3N2))
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3693.05

構造登録者

Thomaston, J.L.,DeGrado, W.F. (登録日: 2015-06-12, 公開日: 2016-05-04, 最終更新日: 2024-11-13)

主引用文献

Thomaston, J.L.,DeGrado, W.F.
Crystal structure of the drug-resistant S31N influenza M2 proton channel.
Protein Sci., 25:1551-1554, 2016

PubMed Abstract: The M2 protein is a small proton channel found in the influenza A virus that is necessary for viral replication. The M2 channel is the target of a class of drugs called the adamantanes, which block the channel pore and prevent the virus from replicating. In recent decades mutations have arisen in M2 that prevent the adamantanes from binding to the channel pore, with the most prevalent of these mutations being S31N. Here we report the first crystal structure of the S31N mutant crystallized using lipidic cubic phase crystallization techniques and solved to 1.59 Å resolution. The Asn31 residues point directly into the center of the channel pore and form a hydrogen-bonded network that disrupts the drug-binding site. Ordered waters in the channel pore form a continuous hydrogen bonding network from Gly34 to His37.

PubMed: 27082171
DOI: 10.1002/pro.2937

実験手法

X-RAY DIFFRACTION (1.591 Å)