5BZW

Crystal Structure of PCDN-27B, an antibody from the PCDN family of HIV-1 antibodies

5BZW の概要

• エントリーDOI: 10.2210/pdb5bzw/pdb
• 関連するPDBエントリー: 5BZD
• 分子名称: 2B3 HIV Antibody light chain, 2B3 HIV Antibody heavy chain, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: hiv, antibody, fab, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49129.56

構造登録者

Garces, F.,WILSON, I.A. (登録日: 2015-06-11, 公開日: 2016-05-25, 最終更新日: 2024-11-20)

主引用文献

MacLeod, D.T.,Choi, N.M.,Briney, B.,Garces, F.,Ver, L.S.,Landais, E.,Murrell, B.,Wrin, T.,Kilembe, W.,Liang, C.H.,Ramos, A.,Bian, C.B.,Wickramasinghe, L.,Kong, L.,Eren, K.,Wu, C.Y.,Wong, C.H.,Kosakovsky Pond, S.L.,Wilson, I.A.,Burton, D.R.,Poignard, P.
Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch.
Immunity, 44:1215-1226, 2016

PubMed Abstract: The high-mannose patch on HIV Env is a preferred target for broadly neutralizing antibodies (bnAbs), but to date, no vaccination regimen has elicited bnAbs against this region. Here, we present the development of a bnAb lineage targeting the high-mannose patch in an HIV-1 subtype-C-infected donor from sub-Saharan Africa. The Abs first acquired autologous neutralization, then gradually matured to achieve breadth. One Ab neutralized >47% of HIV-1 strains with only ∼11% somatic hypermutation and no insertions or deletions. By sequencing autologous env, we determined key residues that triggered the lineage and participated in Ab-Env coevolution. Next-generation sequencing of the Ab repertoire showed an early expansive diversification of the lineage followed by independent maturation of individual limbs, several of them developing notable breadth and potency. Overall, the findings are encouraging from a vaccine standpoint and suggest immunization strategies mimicking the evolution of the entire high-mannose patch and promoting maturation of multiple diverse Ab pathways.

PubMed: 27192579
DOI: 10.1016/j.immuni.2016.04.016

実験手法

X-RAY DIFFRACTION (2.9 Å)