5BXT

LNBase in complex with LNB-NHAcAUS

Summary for 5BXT

• Entry DOI: 10.2210/pdb5bxt/pdb
• Related: 5BXP 5BXR 5BXS
• Descriptor: Lacto-N-biosidase, SULFATE ION, N-{[(1R,2R,3R,7S,7aR)-1,2,7-trihydroxyhexahydro-1H-pyrrolizin-3-yl]methyl}acetamide, ... (5 entities in total)
• Functional Keywords: tim barrel, hydrolase, distal gut, human milk oligosaccharides, inhibitor
• Biological source: Bifidobacterium bifidum JCM 1254
• Total number of polymer chains: 2
• Total formula weight: 145085.60

Authors

Ito, T.,Arakawa, T.,Fushinobu, S. (deposition date: 2015-06-09, release date: 2015-09-09, Last modification date: 2024-11-06)

Primary citation

Hattie, M.,Ito, T.,Debowski, A.W.,Arakawa, T.,Katayama, T.,Yamamoto, K.,Fushinobu, S.,Stubbs, K.A.
Gaining insight into the catalysis by GH20 lacto-N-biosidase using small molecule inhibitors and structural analysis
Chem.Commun.(Camb.), 51:15008-15011, 2015

PubMed Abstract: The synthesis of potent inhibitors for lacto-N-biosidases and X-ray structural characterization of these compounds in complex with BbLNBase is described.

PubMed: 26312778
DOI: 10.1039/c5cc05494j

Experimental method

X-RAY DIFFRACTION (1.8 Å)