5BWI

Crystallographic structure of a bacterial heparanase

5BWI の概要

• エントリーDOI: 10.2210/pdb5bwi/pdb
• 分子名称: Glycoside Hydrolase Family 79, GLYCEROL, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: glucuronidase, hydrolase
• 由来する生物種: Burkholderia pseudomallei
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91089.07

構造登録者

Yu, X.,Blanchard, H. (登録日: 2015-06-08, 公開日: 2015-10-28, 最終更新日: 2024-11-13)

主引用文献

Bohlmann, L.,Tredwell, G.D.,Yu, X.,Chang, C.W.,Haselhorst, T.,Winger, M.,Dyason, J.C.,Thomson, R.J.,Tiralongo, J.,Beacham, I.R.,Blanchard, H.,von Itzstein, M.
Functional and structural characterization of a heparanase.
Nat.Chem.Biol., 11:955-957, 2015

PubMed Abstract: We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.

PubMed: 26565989
DOI: 10.1038/nchembio.1956

実験手法

X-RAY DIFFRACTION (1.6 Å)