5BVI

X-ray Structure of Interferon Regulatory Factor 4 IAD Domain

Summary for 5BVI

• Entry DOI: 10.2210/pdb5bvi/pdb
• Descriptor: Interferon regulatory factor 4, CHLORIDE ION (3 entities in total)
• Functional Keywords: interferon regulatory factors, transcription activation/protein-dna interaction, transcription
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 2
• Total formula weight: 43068.71

Authors

Escalate, C.R.,Remesh, S.G. (deposition date: 2015-06-05, release date: 2015-09-30, Last modification date: 2023-09-27)

Primary citation

Remesh, S.G.,Santosh, V.,Escalante, C.R.
Structural Studies of IRF4 Reveal a Flexible Autoinhibitory Region and a Compact Linker Domain.
J.Biol.Chem., 290:27779-27790, 2015

PubMed Abstract: IRF4 is a unique member of the interferon regulatory factor (IRF) family playing critical regulatory roles in immune cell development, regulation of obesity-induced inflammation, and control of thermogenic gene expression. The ability of IRF4 to control diverse transcriptional programs arises from its proficiency to interact with numerous transcriptional partners. In this study, we present the structural characterization of full-length IRF4. Using a combination of x-ray and small angle x-ray scattering studies, we reveal unique features of the interferon activation domain, including a set of β-sheets and loops that serve as the binding site for PU.1, and also show that unlike other IRF members, IRF4 has a flexible autoinhibitory region. In addition, we have determined the small angle x-ray scattering solution structure of full-length IRF4, which, together with circular dichroism studies, suggests that the linker region is not extended but folds into a domain structure.

PubMed: 26405037
DOI: 10.1074/jbc.M115.678789

Experimental method

X-RAY DIFFRACTION (2.6 Å)